SensoLyte® 520 beta-Secretase (BACE1) Activity Assay Kit

Catalog No. ABIN1882408

Product Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 beta-Secretase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL® 520/ HiLyte Fluor™ 488 FRET substrate. The sequence of this FRET peptide is derived from the beta-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of beta-secretase to process APP, resulting in an early onset of AD. Active beta-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte Fluor™ 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte Fluor™ 488 is less interfered by the autofluorescence of components in biological samples and test compounds.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C, except for Component F. Store b-secretase (Component F) at -80 °C. Protect Components A and B from light and from moisture. Components D and E can be stored at room temperature for convenience.

References

Boddapati, Levites, Sierks: "Inhibiting ?-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP." in: Journal of molecular biology, Vol. 405, Issue 2, pp. 436-47, (2011) (PubMed).

Mowrer, Wolfe: "Promotion of BACE1 mRNA alternative splicing reduces amyloid beta-peptide production." in: The Journal of biological chemistry, Vol. 283, Issue 27, pp. 18694-701, (2008) (PubMed).

Lee, Park, Han: "Effect of mycelial extract of Clavicorona pyxidata on the production of amyloid beta-peptide and the inhibition of endogenous beta-secretase activity in vitro." in: Journal of microbiology (Seoul, Korea), Vol. 44, Issue 6, pp. 665-70, (2007) (PubMed).

Target Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1 (BACE1 Products)

Synonyms: ASP2 Kit, BACE Kit, HSPC104 Kit, C76936 Kit, Bace Kit, MGC145931 Kit, BACE1 Kit, zgc:77409 Kit, beta-secretase 1 Kit, beta-site APP cleaving enzyme 1 Kit, beta-site APP-cleaving enzyme 1 Kit, BACE1 Kit, Bace1 Kit, bace1 Kit

Background: Beta-Secretase is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing beta-amyloid peptides through proteolytic cleavage of the beta-amyloid precursor protein (APP). Accumulation of neurotoxic beta-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer's disease (AD), an age-related cognitive disorder. Therefore, targeting beta-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD.