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- Target See all ADAM10 Kits
- ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
- Detection Method
- Fluorometric
- Application
- Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® 520 ADAM10 Activity Assay Kit is an optimized assay that can be used to detect the ADAM 10 activity. The unique FRET substrate contained in the kit was designed to reduce the cross reactivity with ADAM17 (also called tumor necrosis factor-alpha-converting enzyme, TACE). When active ADAM10 cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.25 ng/mL active ADAM10.
- Top Product
- Discover our top product ADAM10 ELISA Kit
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ADAM Metallopeptidase Domain 10 (ADAM10) ELISA Kit
ADAM10 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 10 (ADAM10) ELISA KitADAM10 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 10 (ADAM10) ELISA KitADAM10 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
ADAM Metallopeptidase Domain 10 (ADAM10) ELISA KitADAM10 Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
ADAM Metallopeptidase Domain 10 (ADAM10) ELISA KitADAM10 Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 10 (ADAM10) ELISA KitHigh Sensitivity ADAM10 Reactivity: Mouse Colorimetric Sandwich ELISA 9.4 pg/mL - 600 pg/mL Plasma, Serum, Tissue Homogenate
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- Comment
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FRET-based Assay Kit
- Restrictions
- For Research Use only
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- Handling Advice
- Protect components A and B from light and moisture.
- Storage
- -80 °C
- Storage Comment
- Store all kit components at -20 °C except component C, which should be stored at -80 °C. Component D can be stored at RT for convenience.
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- Target
- ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
- Alternative Name
- ADAM10 (ADAM10 Products)
- Background
- ADAM10 has been shown to be an effective alpha-secretase in vitro and in vivo. This enzyme acts as a sheddase to cleave cell surface proteins involved in neuropathology, inflammatory response and tumor progression.
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