SensoLyte® 390 ACE2 Activity Assay Kit

Catalog No. ABIN1882389

Product Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 390 ACE2 Activity Assay Kit provides a convenient assay for high throughput screening of ACE2 enzyme inhibitors and inducers using a Mc-Ala/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of Mc-Ala is quenched by Dnp. Upon cleavage into two separate fragments by ACE2, the fluorescence of Mc-Ala is recovered, and can be monitored at excitation/emission = 330/390 nm. This assay can detect the activity of subnanogram level of ACE2. Assays are performed in a convenient 96-well microplate format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light and from moisture.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components C and E can be stored at room temperature for convenience.

References

Liu, Liu, Miura, Tanabe, Maeda, Terayama, Turner, Zou, Komano: "Conversion of A?43 to A?40 by the successive action of angiotensin-converting enzyme 2 and angiotensin-converting enzyme." in: Journal of neuroscience research, Vol. 92, Issue 9, pp. 1178-86, (2014) (PubMed).

Raffai, Khang, Vanhoutte: "Angiotensin-(1-7) augments endothelium-dependent relaxations of porcine coronary arteries to bradykinin by inhibiting angiotensin-converting enzyme 1." in: Journal of cardiovascular pharmacology, Vol. 63, Issue 5, pp. 453-60, (2014) (PubMed).

Gao, Yallampalli, Yallampalli: "Gestational protein restriction increases angiotensin II production in rat lung." in: Biology of reproduction, Vol. 88, Issue 3, pp. 64, (2013) (PubMed).

Luhtala, Vaajanen, Oksala, Valjakka, Vapaatalo: "Activities of angiotensin-converting enzymes ACE1 and ACE2 and inhibition by bioactive peptides in porcine ocular tissues." in: Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, Vol. 25, Issue 1, pp. 23-8, (2009) (PubMed).

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2 (ACE2 Products)

Synonyms: ACE1 Kit, CD143 Kit, DCP Kit, DCP1 Kit, ICH Kit, MVCD3 Kit, ACEH Kit, 2010305L05Rik Kit, zgc:92514 Kit, angiotensin I converting enzyme Kit, angiotensin I converting enzyme 2 Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Kit, ACE Kit, ACE2 Kit, Ace2 Kit, ace2 Kit

Background: ACE2 or ACEH, angiotensin-converting enzyme 2, a homolog of angiotensin-converting enzyme, is a novel zinc metallopeptidase with specificity, tissue distribution, and function distinct from those of ACE. ACE2 may play a unique role in the renin-angiotensin system and mediate cardiovascular and renal function.