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ICAM2 ELISA Kit

ICAM2 Reactivity: Human AA 25-223 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Catalog No. ABIN1672894
  • Target See all ICAM2 ELISA Kits
    ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))
    Binding Specificity
    AA 25-223
    Reactivity
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    312-20000 pg/mL
    Minimum Detection Limit
    312 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ICAM-2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: K25-Q223
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: K25-Q223
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the immunoglobulin superfamily. ICAM family.

    Plate
    Pre-coated
    Protocol
    human ICAM-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-2 has been precoated onto 96-well plates. Standards(NSO, K25-Q223) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ICAM-2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ICAM-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ICAM-2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.066, CV(%): 4.2
    • Sample 2: n=16, Mean(ng/ml): 7.62, Standard deviation: 0.343, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 13.3, Standard deviation: 0.705, CV(%): 5.3,
    • Sample 1: n=24, Mean(ng/ml): 1.36, Standard deviation: 0.078, CV(%): 5.7
    • Sample 2: n=24, Mean(ng/ml): 7.53, Standard deviation: 0.152, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 14.8, Standard deviation: 1.11, CV(%): 7.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-?-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, (2011) (PubMed).

  • Target See all ICAM2 ELISA Kits
    ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))
    Alternative Name
    ICAM2 (ICAM2 Products)
    Synonyms
    ICAM2 ELISA Kit, CD102 ELISA Kit, Icam-2 ELISA Kit, Ly-60 ELISA Kit, intercellular adhesion molecule 2 ELISA Kit, LOC506088 ELISA Kit, ICAM2 ELISA Kit, LOC616254 ELISA Kit, LOC100140873 ELISA Kit, Icam2 ELISA Kit
    Background

    Protein Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

    Background: Intercellular adhesion molecule 2(ICAM2), also known as CD102(Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

    Synonyms: Intercellular adhesion molecule 2,ICAM-2,CD102,ICAM2,

    Full Gene Name: Intercellular adhesion molecule 2

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    3384
    UniProt
    P13598
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