IL-22 ELISA Kit

Catalog No. ABIN1672846

Product Details IL-22 ELISA Kit

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Binding Specificity: AA 34-179

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-22

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A34-I179

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A34-I179

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the IL-10 family.

Plate: Pre-coated

Protocol: human IL-22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-22 has been precoated onto 96-well plates. Standards(E.coli, A34-I179) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-22 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-22 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-22 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 74, Standard deviation: 3.63, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 312, Standard deviation: 18.1, CV(%): 5.8
• Sample 3: n=16, Mean(pg/ml): 611, Standard deviation: 37.3, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 87, Standard deviation: 4.7, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 386, Standard deviation: 25.5, CV(%): 6.6
• Sample 3: n=24, Mean(pg/ml): 695, Standard deviation: 48, CV(%): 6.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-22 ELISA Kit (ABIN1672846)

Min, Yan, Wang, Landeck, Chen, Li, Cai, Zheng, Man: "Rottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies." in: PLoS ONE, Vol. 12, Issue 12, pp. e0190051, (2018) (PubMed).

Chai, Yang, Gao, Guo, Li, Fan, Wu, Xue, Cai, Jiang, Qin, Zhang, Ke: "Decreased percentages of regulatory T cells are necessary to activate Th1-Th17-Th22 responses during acute rejection of the peripheral nerve xenotransplantation in mice." in: Transplantation, Vol. 98, Issue 7, pp. 729-37, (2014) (PubMed).

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL22 (IL22 Products)

Synonyms: IL-21 ELISA Kit, IL-22 ELISA Kit, IL-D110 ELISA Kit, IL-TIF ELISA Kit, ILTIF ELISA Kit, TIFIL-23 ELISA Kit, TIFa ELISA Kit, zcyto18 ELISA Kit, IL-22a ELISA Kit, ILTIFa ELISA Kit, Iltif ELISA Kit, RGD1561292 ELISA Kit, interleukin-22 ELISA Kit, interleukin 22 ELISA Kit, IL22 ELISA Kit, il22 ELISA Kit, Il22 ELISA Kit

Background:

Protein Function: Cytokine that contributes to the inflammatory response in vivo.

Background: Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Synonyms: Interleukin-22,IL-22,Cytokine Zcyto18,IL-10-related T-cell-derived-inducible factor,IL-TIF,IL22,ILTIF, ZCYTO18,UNQ3099/PRO10096,

Full Gene Name: Interleukin-22

Cellular Localisation: Secreted.

Gene ID: 50616

UniProt: Q9GZX6