CD86 ELISA Kit

Catalog No. ABIN1672778

Product Details CD86 ELISA Kit

Target: CD86

Binding Specificity: AA 29-250

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD86/B7-2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: V29-I250

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: V29-I250

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD86 has been precoated onto 96-well plates. Standards(NSO, V29-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD86 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD86 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CD86 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.21, Standard deviation: 0.010, CV(%): 4.8
• Sample 2: n=16, Mean(ng/ml): 1.44, Standard deviation: 0.102, CV(%): 7.1
• Sample 3: n=16, Mean(ng/ml): 2.59, Standard deviation: 0.161, CV(%): 6.2,
• Sample 1: n=24, Mean(ng/ml): 0.23, Standard deviation: 0.013, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.118, CV(%): 8.0
• Sample 3: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.182, CV(%): 7.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CD86

Target: CD86

Alternative Name: CD86 (CD86 Products)

Synonyms: B7-2 ELISA Kit, B7.2 ELISA Kit, B70 ELISA Kit, CD28LG2 ELISA Kit, LAB72 ELISA Kit, B7 ELISA Kit, CLS1 ELISA Kit, Cd28l2 ELISA Kit, ETC-1 ELISA Kit, Ly-58 ELISA Kit, Ly58 ELISA Kit, MB7 ELISA Kit, MB7-2 ELISA Kit, TS/A-2 ELISA Kit, CD86 molecule ELISA Kit, CD86 antigen ELISA Kit, integrin subunit alpha 2b ELISA Kit, CD86 ELISA Kit, Cd86 ELISA Kit, Itga2b ELISA Kit

Background:

Background: Cluster of Differentiation 86(also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Synonyms: B7-2 ,Cd86 ,

Full Gene Name: CD86 Molecule

Gene ID: 56822

UniProt: Q62810

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation