CD80 ELISA Kit

Catalog No. ABIN1672777

Product Details CD80 ELISA Kit

Target: CD80

Binding Specificity: AA 35-242

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human B7-1/CD80

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: V35-N242

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: V35-N242

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells.

Plate: Pre-coated

Protocol: human CD80 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, V35-N242) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD80 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD80 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.013, CV(%): 5.7
• Sample 2: n=16, Mean(ng/ml): 1.47, Standard deviation: 0.090, CV(%): 6.1
• Sample 3: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.185, CV(%): 7.9,
• Sample 1: n=24, Mean(ng/ml): 0.27, Standard deviation: 0.018, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 1.56, Standard deviation: 0.115, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 2.58, Standard deviation: 0.214, CV(%): 8.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CD80

Target: CD80

Alternative Name: CD80 (CD80 Products)

Synonyms: B7 ELISA Kit, B7-1 ELISA Kit, B7.1 ELISA Kit, BB1 ELISA Kit, CD28LG ELISA Kit, CD28LG1 ELISA Kit, LAB7 ELISA Kit, B71 ELISA Kit, Cd28l ELISA Kit, Ly-53 ELISA Kit, Ly53 ELISA Kit, MIC17 ELISA Kit, TSA1 ELISA Kit, CD80 molecule ELISA Kit, Cd80 molecule ELISA Kit, CD80 antigen ELISA Kit, CD80 ELISA Kit, Cd80 ELISA Kit

Background:

Protein Function: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .

Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Synonyms: T-lymphocyte activation antigen CD80,Activation B7-1 antigen,BB1,CTLA-4 counter-receptor B7.1,B7,CD80,CD80,CD28LG, CD28LG1, LAB7,

Full Gene Name: T-lymphocyte activation antigen CD80

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 941

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints