Prolactin ELISA Kit

Catalog No. ABIN1672760

Product Details Prolactin ELISA Kit

Target: Prolactin (PRL)

Binding Specificity: AA 29-227

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Prolactin

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: L29-C227

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: L29-C227

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the somatotropin/prolactin family.

Plate: Pre-coated

Protocol: human Prolactin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Prolactin has been precoated onto 96-well plates. Standards(E.coli, L29-C227) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prolactin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Prolactin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Prolactin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Prolactin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1491, Standard deviation: 85, CV(%): 5.7
• Sample 2: n=16, Mean(pg/ml): 2734, Standard deviation: 134, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 4962, Standard deviation: 248.1, CV(%): 5,
• Sample 1: n=24, Mean(pg/ml): 1529, Standard deviation: 96.33, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 2850, Standard deviation: 196.7, CV(%): 6.9
• Sample 3: n=24, Mean(pg/ml): 5271, Standard deviation: 390, CV(%): 7.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Prolactin ELISA Kit (ABIN1672760)

Garrido-Gomez, Dominguez, Quiñonero, Diaz-Gimeno, Kapidzic, Gormley, Ona, Padilla-Iserte, McMaster, Genbacev, Perales, Fisher, Simón: "Defective decidualization during and after severe preeclampsia reveals a possible maternal contribution to the etiology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 40, pp. E8468-E8477, (2018) (PubMed).

Wu, Kimura, Zheng, Ishida, Niwa, Hirata, Takebayashi, Takashima, Takahashi, Kushima, Zhang, Murakami: "Chronic endometritis modifies decidualization in human endometrial stromal cells." in: Reproductive biology and endocrinology : RB&E, Vol. 15, Issue 1, pp. 16, (2017) (PubMed).

Xia, Qi, Zou, Yao: "Influenza A(H1N1) vaccination during early pregnancy transiently promotes hippocampal neurogenesis and working memory. Involvement of Th1/Th2 balance." in: Brain research, Vol. 1592, pp. 34-43, (2015) (PubMed).

Target Details for Prolactin

Target: Prolactin (PRL)

Alternative Name: PRL (PRL Products)

Background:

Protein Function: Prolactin acts primarily on the mammary gland by promoting lactation.

Background: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.

Synonyms: Prolactin,PRL,PRL,

Full Gene Name: Prolactin

Cellular Localisation: Secreted.

Gene ID: 5617

UniProt: P01236

Pathways: JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus