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CCL24 ELISA Kit

CCL24 Reactivity: Mouse AA 27-119 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1672758
  • Target See all CCL24 ELISA Kits
    CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))
    Binding Specificity
    AA 27-119
    Reactivity
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL24/Eotaxin-2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: V27-V119
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: V27-V119
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis. .

    Plate
    Pre-coated
    Protocol
    mouse CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli, V27-V119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL24 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CCL24 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CCL24 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 125, Standard deviation: 6, CV(%): 4.8
    • Sample 2: n=16, Mean(pg/ml): 257, Standard deviation: 10.79, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 526, Standard deviation: 33.14, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 130, Standard deviation: 10.14, CV(%): 7.8
    • Sample 2: n=24, Mean(pg/ml): 249, Standard deviation: 15.94, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 42.99, CV(%): 8.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CCL24 ELISA Kits
    CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))
    Alternative Name
    CCL24 (CCL24 Products)
    Synonyms
    Ckb-6 ELISA Kit, MPIF-2 ELISA Kit, MPIF2 ELISA Kit, SCYA24 ELISA Kit, CKb-6 ELISA Kit, Scya24 ELISA Kit, C-C motif chemokine ligand 24 ELISA Kit, chemokine (C-C motif) ligand 24 ELISA Kit, CCL24 ELISA Kit, Ccl24 ELISA Kit
    Background

    Protein Function: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Binds to CCR3 (By similarity). .

    Background: Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

    Synonyms: C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24,

    Full Gene Name: C-C motif chemokine 24

    Cellular Localisation: Secreted.
    Gene ID
    56221
    UniProt
    Q9JKC0
    Pathways
    Regulation of Actin Filament Polymerization
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