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CCL19 ELISA Kit

CCL19 Reactivity: Human AA 22-98 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1672744
  • Target See all CCL19 ELISA Kits
    CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))
    Binding Specificity
    AA 22-98
    Reactivity
    • 9
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL19/MIP-3 beta
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: G22-S98
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: G22-S98
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    human CCL19 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL19 has been precoated onto 96-well plates. Standards(E.coli, G22-S98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL19 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL19 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL19 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 97, Standard deviation: 5.53, CV(%): 5.7
    • Sample 2: n=16, Mean(pg/ml): 293, Standard deviation: 14.1, CV(%): 4.8
    • Sample 3: n=16, Mean(pg/ml): 583, Standard deviation: 36.15, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 117, Standard deviation: 7.5, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 374, Standard deviation: 18.7, CV(%): 5
    • Sample 3: n=24, Mean(pg/ml): 627, Standard deviation: 42.64, CV(%): 6.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CCL19 ELISA Kits
    CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))
    Alternative Name
    CCL19 (CCL19 Products)
    Synonyms
    CKb11 ELISA Kit, ELC ELISA Kit, MIP-3b ELISA Kit, MIP3B ELISA Kit, SCYA19 ELISA Kit, CCL19 ELISA Kit, Scya19 ELISA Kit, exodus-3 ELISA Kit, C-C motif chemokine ligand 19 ELISA Kit, chemokine (C-C motif) ligand 19 ELISA Kit, CCL19 ELISA Kit, ccl19 ELISA Kit, Ccl19 ELISA Kit
    Background

    Background: Chemokine(C-C motif) ligand 19(CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine(ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

    Synonyms: C-C motif chemokine ,CCL19 ,hCG_20476 ,

    Full Gene Name: C-C motif chemokine

    Cellular Localisation: Secreted.
    Gene ID
    6363
    UniProt
    Q6IBD6
    Pathways
    Positive Regulation of Immune Effector Process
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