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CXCL6 ELISA Kit

CXCL6 Reactivity: Human AA 40-114 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1672728
  • Target See all CXCL6 ELISA Kits
    CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))
    Binding Specificity
    AA 40-114
    Reactivity
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL6/GCP2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: V40-N114
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: V40-N114
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

    Plate
    Pre-coated
    Protocol
    human CXCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL6 has been precoated onto 96-well plates. Standards(E.coli, V40-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL6 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL6 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 223, Standard deviation: 13.6, CV(%): 6.1
    • Sample 2: n=16, Mean(pg/ml): 519, Standard deviation: 33.2, CV(%): 6.4
    • Sample 3: n=16, Mean(pg/ml): 1089, Standard deviation: 64.3, CV(%): 5.9,
    • Sample 1: n=24, Mean(pg/ml): 245, Standard deviation: 18.1, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 542, Standard deviation: 41.2, CV(%): 7.6
    • Sample 3: n=24, Mean(pg/ml): 1054, Standard deviation: 71.7, CV(%): 6.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CXCL6 ELISA Kits
    CXCL6 (Chemokine (C-X-C Motif) Ligand 6 (CXCL6))
    Alternative Name
    CXCL6 (CXCL6 Products)
    Synonyms
    CKA-3 ELISA Kit, GCP-2 ELISA Kit, GCP2 ELISA Kit, SCYB6 ELISA Kit, Cxcl6 ELISA Kit, CXCL5 ELISA Kit, C-X-C motif chemokine ligand 6 ELISA Kit, chemokine (C-X-C motif) ligand 5 ELISA Kit, CXCL6 ELISA Kit, Cxcl6 ELISA Kit, CXCL5 ELISA Kit
    Background

    Protein Function: Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram- negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7). .

    Background: Chemokine(C-X-C motif) ligand 6(CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2(GCP-2). As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.

    Synonyms: C-X-C motif chemokine 6,Chemokine alpha 3,CKA-3,Granulocyte chemotactic protein 2,GCP-2,Small-inducible cytokine B6,Small-inducible cytokine B6, N-processed variant 1,Small-inducible cytokine B6, N-processed variant 2,Small-inducible cytokine B6, N-processed variant 3,CXCL6,GCP2, SCYB6,

    Full Gene Name: C-X-C motif chemokine 6

    Cellular Localisation: Secreted.
    Gene ID
    6372
    UniProt
    P80162
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