Cathepsin D ELISA Kit

Catalog No. ABIN1569245

Product Details Cathepsin D ELISA Kit

Target: Cathepsin D (CTSD)

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 3.125 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.125 ng/mL

Application: ELISA

Purpose: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CTSD in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of this index.

Cross-Reactivity (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.88 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Material not included:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Sample Volume: 50 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Assay Procedure:

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

Assay Precision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Expiry Date: 12 months

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: CTSD (CTSD Products)

Synonyms: catD ELISA Kit, fb93e11 ELISA Kit, fj17b09 ELISA Kit, wu:fb93e11 ELISA Kit, wu:fj17b09 ELISA Kit, LOC398557 ELISA Kit, Cathd ELISA Kit, CatD ELISA Kit, LOC100136761 ELISA Kit, CTSD ELISA Kit, DKFZp469J0315 ELISA Kit, DDBDRAFT_0205785 ELISA Kit, DDBDRAFT_0215012 ELISA Kit, DDB_0205785 ELISA Kit, DDB_0215012 ELISA Kit, CLN10 ELISA Kit, CPSD ELISA Kit, CD ELISA Kit, cathepsin D ELISA Kit, cathepsin D S homeolog ELISA Kit, lysosomal aspartic protease ELISA Kit, cathepsin D-like ELISA Kit, ctsd ELISA Kit, LOC398557 ELISA Kit, ctsd.S ELISA Kit, CatD ELISA Kit, LOC5567565 ELISA Kit, LOC100136761 ELISA Kit, CTSD ELISA Kit, Cathd ELISA Kit, ctsD ELISA Kit, catd ELISA Kit, Ctsd ELISA Kit, LOC443060 ELISA Kit

Background: Alternative name: CPSD, CLN10, Lysosomal Aspartyl Protease, Ceroid-Lipofuscinosis,Neuronal 10

Gene ID: 282883

UniProt: F1MMR6

Pathways: Peptide Hormone Metabolism