CMV IgM ELISA Kit
CMV IgM
Reactivity: Human
Colorimetric
Competition ELISA
Plasma, Serum
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- Target See all CMV IgM products
- CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Purpose
- CMV IgM ELISA Kit is intended for the detection of IgM antibody to Cytomegalovirus (CMV) in human serum or plasma.
- Sample Type
- Plasma, Serum
- Analytical Method
- Qualitative
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- Application Notes
- Diluted patient serum (serum diluent contains sorbent to remove Rheumatoid Factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample.
- Plate
- Pre-coated
- Reagent Preparation
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Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25°C).
- Calculation of Results
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- Check Calibrator Factor (CF) value on the calibrator bottle. This value might vary from lot to lot. Make sure you check the value on every kit.
- Calculate the cut-off value: Calibrator OD x Calibrator Factor (CF).
- Calculate the Ab (Antibody) Index of each determination by dividing the O.D. value of each sample by cut-off value.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all CMV IgM products
- CMV IgM (Anti-Cytomegalovirus IgM Antibody (CMV IgM))
- Alternative Name
- CMV IgM (CMV IgM Products)
- Target Type
- Antibody
- Background
- Cytomegalovirus (CMV) is a member of the herpes group of viruses. Most adults and children who catch CMV have no symptoms and are not harmed by the virus. CMV infection is of clinical significance primarily in pregnant women, newborn infants with possible congenital infection, immunosuppressed transplant patients and iniduals with AIDS. CMV is so prevalent as over 60% of people catch the infection at some time in their lives.Significant increases in CMV IgG antibody by ELISA suggest recent infection or reactivation of a latent CMV infection.CMV IgM antibody can be detected by ELISA in both primary CMV infections (93-100%) and in reactivated infection (40%). An IgM response may be reduced or absent in immunocompromised patients with active infection. In transplant patients the CMV infection can be associated with higher morbidity and mortality.
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