Anti-Cardiolipin Antibody ELISA Kit

Catalog No. ABIN1326805

Product Details Anti-Cardiolipin Antibody ELISA Kit

Target: Anti-Cardiolipin Antibody (ACA)

Binding Specificity: Whole Molecule

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Purpose: Diluted patient serum is added to wells coated with purified aCL antigen. aCL specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of specific antibody in the sample.

Sample Type: Serum

Analytical Method: Qualitative

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for Anti-Cardiolipin Antibody ELISA Kit (ABIN1326805)

Rupasree, Naushad, Rajasekhar, Uma, Kutala: "Association of TLR4 (D299G, T399I), TLR9 -1486T>C, TIRAP S180L and TNF-? promoter (-1031, -863, -857) polymorphisms with risk for systemic lupus erythematosus among South Indians." in: Lupus, Vol. 24, Issue 1, pp. 50-7, (2014) (PubMed).

Target Details for Anti-Cardiolipin Antibody

Target: Anti-Cardiolipin Antibody (ACA)

Abstract: ACA Products

Target Type: Antibody

Background: Measurement of IgG, IgM and IgA cardiolipin autoantibodies (aCL) by EIA is the standard procedure for the detection of antiphospholipid antibodies (aPL) in patients with suspected antiphospholipid syndrome (APS).High aCL concentrations are associated with increased risk of venous and arterial thrombosis, recurrent pregnancy loss and thrombocytopenia. Patients with the anti-cardiolipin syndrome have one of the above clinical features and have antibodies to cardiolipin andor a positive lupus anticoagulant test. The antibodies present to cardiolipin may be of the IgG, IgA, IgM isotypes.Testing for the various antibody isotypes to cardiolipin aid in diagnosis of the anti-phospholipid syndrome in patients with SLE or lupus-like disorders. Binding of aCL to CL in patients with autoimmune diseases is dependent on the presence of the cofactor ibetai-2-glycoprotein I (ibetasub2subi-GPI) this binding is independent of ibetai-2-GPI in patients with infectious diseases (e.g., syphilis, tuberculosis). Recognition of the role of ibetai-2-GPI in the binding of aCL led to development of assay for direct measurement of ibetai-2-GPI autoantibodies using ibetai-2-GPI as antigen,allowing a clear distinction between ibetai-2-GPI autoantibodies and those that bind to CL alone.