Fecal Calprotectin Strip Test Kit

Catalog No. ABIN1305191

Product Details

Target: Calprotectin (S100A8/A9)

Reactivity: Human

Application: Rapid Test (rapid)

Purpose: The Calprotectin Strip Test is a ?sandwich? immunoassay utilizing two monoclonal antibodies to specifically detect the presence of Calprotectin in feces. The kit consists of two components, a fecal sampling extraction buffer and a test strip. In this test, the specimen is first treated with an extraction solution to extract calprotectin from the feces in a test tube. Following extraction, the only remaining step required is to dip the calprotectin test strip into the fecal suspension in the test tube. As the sample extraction reaches the test strip, the colored particles migrate. In the case of a positive result, the specific antibodies present on the membrane will capture the colored particles. Different colored lines will be visible, depending upon the calprotectin content of the sample. After 5 minutes of incubation at room temperature, test lines will appear to interpret the result. If the Calprotectin level is greater than 50 ?g/g in a fecal sample extract, an immuno-complex of ?labeled monoclonal anti-calprotectin antibody ? membrane coated monoclonal anti-human calprotectin antibody? is formed. A red colored band appears in the test region, which is located in the lower half of the test membrane. A similar colored band must appear in the control region located in the upperhalf of the test membrane, indicating that the test strip is functioning properly and the result is valid.

Sample Type: Fecal

Analytical Method: Qualitative

Cross-Reactivity (Details): this Calprotectin rapid test does not cross-react to human albumin, hemoglobin.

Components: 1. Calprotectin Test Strip,
2. Fecal Sample Extraction Buffer, 50 mL
3. Instruction for use 1

Material not included: 1. Disposable pipette for watery sample collection
2. Plastic or glass test tube (13x75 mm or similar size)
3. 10 µL inoculating loop
4. Positive Control

Application Details

Assay Time: 0.5 h

Protocol: This Calprotectin Strip Test is a rapid immunological test intended for the qualitative detection of Calprotectin in feces by professional laboratories and physician office laboratories. This test is used as an aid for the diagnosis of inflammatory bowel disease. Fecal Calprotectin levels correlate significantly with histological and endoscopic assessment of disease activity in ulcerative collitis, as well as with fecal -antitrypsin levels and fecal excretion of indium labeled white blood cells in patients with Crohn disease.

Sample Collection: 1. Stool specimens can be collected at any time of the day.
2. Collect a random sample of feces into a fecal sample collection container or tube or cup with an aid of a clean, dry cup or toilet paper.
3. The specimen is ready for testing, transportation or storage. It can be stored at 2-8 °C for up to 14 days and at room temperature for up to 5 days.

Assay Procedure:

Line up and mark test tubes to match the number of samples to be tested. 2. Add 0.5 mLor 12 drops of the Fecal Sample Extraction Buffer to each tube. 3. For each solid specimen: collect one loop of stool sample and insert the inoculating loop containing the stool sample into a tube. For each liquid specimen: collect totally three loops of stool sample and dip the inoculating loop containing the stool sample three times into a tube. 4. Mix the sampling tube vigorously to ensure a good liquid suspension. Let the mixed sample extract stand for 1-2 minutes. 5. Discard the inoculating loop and immerse one test strip into each tube in the direction indicated by arrow on each test strip. 6. Read test result at 5 minutes and do not interpret test result after 10 minutes.

Calculation of Results:

The appearance of control line on the test reading membrane area indicates the test is valid. The control line located at the upper half of the test membrane area.
1. Positive: If two red/pink colored bands are visible within 10 minutes, the test result is positive and valid.
2. Negative: If test area has no colored band and the control area displays a red/pink colored band, the test result is negative (see figure as Valid Test).

Restrictions: For Research Use only

Handling

Precaution of Use: 1. For in-vitro diagnostic use only. Not to be taken internally. 2. Do not use product beyond the expiration date. 3. Handle all specimens as potentially infectious. 4. Do not reuse the test.

Storage: 4 °C

Target Details

Target: Calprotectin (S100A8/A9)

Alternative Name: Calprotectin (S100A8/A9 Products)

Background: The human Calprotectin (MRP 8/14) is 36 kDa calcium and zinc biding heterodimer expressed by the gene S100 family. It is present in the cytoplasm of neutrophils and expressed by the membranes of monocytes. It constitutes nearly 60 % of the soluble cytosol proteins in neutrophils. Calprotectin is released in the body and may be detected in serum, body fluids, or stool as a potentially useful clinical inflammatory marker for diseases such as Inflammatory Bowel Disease (IBD). IBD is a group of conditions that cause a pathological inflammation of the bowel wall. The main diseases that cause increased excretion of fecal calprotectin are Crohn's disease, ulcerative colitis and neoplasm.