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CPS1 ELISA Kit

CPS1 Reactivity: Human Mitochondrial Colorimetric
Catalog No. ABIN1125223
  • Target See all CPS1 ELISA Kits
    CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))
    Binding Specificity
    Mitochondrial
    Reactivity
    • 3
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Application
    ELISA
    Characteristics
    Homo sapiens,Human,Carbamoyl-phosphate synthase [ammonia], mitochondrial,Carbamoyl-phosphate synthetase I,CPSase I,CPS1,6.3.4.16
    Components
    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
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  • Comment

    Gene Name: CPS1

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Validation #103213 (ELISA)
    'Independent Validation' Badge
    by
    Omary Lab, Department of Molecular & Integrative Physiology, University of Michigan Medical School
    No.
    #103213
    Date
    06/11/2018
    Antigen
    CPS1
    Lot Number
    7K295N
    Method validated
    ELISA
    Positive Control
    Human serum from patients with liver injury
    Negative Control
    Human serum from patients without liver injury
    Notes

    Passed, the CPS1 ELISA kit ABIN1125223 specifically detects the antigen in human serum samples.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    Secondary Antibody
    Full Protocol
    • Freeze human serum samples in aliquots until use. Dilute sera with PBS.
    • Allow all reagents to reach RT and prepare all the reagents, standard solutions and samples as instructed by the manufacturer.
    • Add 100µl of standard, blank or sample per well. Cover the plate. Incubate for 2h at 37°C.
    • Remove the liquid.
    • Add 100µl detection reagent A. Mix gently and cover the plate. Incubate for 1h at 37°C.
    • Aspirate liquid and wash the wells 3x with 400µl wash buffer. Let it sit for 1-2min. Remove liquid Completely at each step.
    • Add 100µl of detection reagent B. Cover the plate. Incubate for 1h at 37°C.
    • Wash well 5x with 400µl wash buffer.
    • Add 90µl substrate solution. Cover the plate. Incubate 15min at 37°C protected from light.
    • Add 50µl stop solution. Gently tap the plate to ensure thorough mixing.
    • Read absorbance at 450nm immediately.
    Experimental Notes
    • The purpose of this report is to validate the measurement of human CPS1 in human serum samples, since it has been published that CPS1 is released into serum during liver injury, while it is not detected in healthy human serum (Weerasinghe et al. (2014)).

    • To validate this ELISA, human serum samples previously validated to contain CPS1 by Western blotting were used. The signal in the positive controls samples (patients’ samples that contain high amount of CPS1 which was confirmed by Western blotting) was significantly higher than the blank whereas the signal from the negative control samples (healthy human sera in that CPS1 was not detected by Western blotting) was comparable to the baseline. 1:5, 1:25, or 1:125 diluted serum samples were tested as well.

    • CPS1 was not detected in the sera of healthy human by both western blotting and ELISA method (lane 1 and 2). However, it is highly increased in the sera of patients with acute liver failure and the signals were in a dilution-dependent manner (lane 3 to 11).

    • The standard curve for the assays reported were fitted with a 4-Parameter Logistic model. The calculated concentrations of standards were higher than expected, but it gave a good quality fits (R2=0.999). The results showed no CPS1 in the sera from healthy human, while it is highly increased in the sera from patients with liver injury, as expected. Although most of the samples were in the detection range, the calculated concentrations were highest in the most diluted samples.

  • Storage
    4 °C/-20 °C
    Storage Comment
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Target See all CPS1 ELISA Kits
    CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))
    Alternative Name
    CPS1 (CPS1 Products)
    Synonyms
    CPSASE1 ELISA Kit, cps ELISA Kit, 4732433M03Rik ELISA Kit, CPS ELISA Kit, D1Ucla3 ELISA Kit, Cps1 ELISA Kit, carbamoyl-phosphate synthase 1 ELISA Kit, carbamoyl-phosphate synthase 1 L homeolog ELISA Kit, carbamoyl-phosphate synthetase 1 ELISA Kit, peptidase M20 domain containing 1 ELISA Kit, CPS1 ELISA Kit, Cps1 ELISA Kit, cps1.L ELISA Kit, cps1 ELISA Kit, PM20D1 ELISA Kit
    Background
    Synonyms: Carbamoyl-phosphate synthase [ammonia], mitochondrial,Carbamoyl-phosphate synthetase I,CPS1,CPSase I,Homo sapiens,Human
    Pathways
    Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process
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