Immunoglobulin isotyping

Catalog No. ABIN1113186

Product Details

Target: Ig

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Detection Method: Colorimetric

Conjugate: HRP

Application: Isotyping (IsoT)

Purpose: The method employs the quantitative sandwich enzyme immunoassay technique.

Brand: FastELISA®

Sample Type: Cell Culture Supernatant, Purified Protein

Specificity: Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,

Characteristics: The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.

Application Details

Comment:

One Step ELISA 30 min total time

Assay Time: < 1 h

Plate: Pre-coated

Protocol: Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Reagent Preparation:

dilution

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Ig

Alternative Name: Immunoglobulins (Ig Products)

Synonyms: ATPIG Kit, ATPIQ Kit, A330005H02Rik Kit, AI315324 Kit, Ig Kit, ATPase phospholipid transporting 11C Kit, ATPase, class VI, type 11C Kit, ATP11C Kit, Atp11c Kit

Background: Isotyping of mouse immunoglobulins