Fetuin A ELISA Kit

Catalog No. ABIN1112602

Product Details Fetuin A ELISA Kit

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: For quantitative detection of Fetuin A in tissue lysates or cell culture supernates.

Sample Type: Tissue Lysate, Cell Culture Supernatant

Analytical Method: Quantitative

Sensitivity: < 10 pg/mL

Components: 1. One 96-well plate pre-coated with anti-Human Fetuin A antibody 2. Lyophilized Human Fetuin A standards: 2 tubes (20ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human Fetuin A antibody (Concentrated): 130 µl.

Material not included: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Application Details

Comment:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Fetuin A polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fetuin A polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Fetuin A amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Fetuin A can be calculated.

Plate: Pre-coated

Reagent Preparation:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Sample Preparation:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, EDTA and citrate as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. For eliminating the platelet effect, suggesting that further centrifugation for 10 min at 2-8°C at 10000 x g. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide, Thimerosal (Merthiolate)

Target Details for Fetuin A

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Alternative Name: Fetuin A (AHSG Products)

Synonyms: ahs ELISA Kit, a2hs ELISA Kit, hsga ELISA Kit, fetua ELISA Kit, wu:fb63g02 ELISA Kit, zgc:103687 ELISA Kit, AHSG ELISA Kit, MGC116429 ELISA Kit, A2HS ELISA Kit, AHS ELISA Kit, FETUA ELISA Kit, HSGA ELISA Kit, Aa2-066 ELISA Kit, pp63 ELISA Kit, alpha-2-HS-glycoprotein ELISA Kit, alpha 2-HS glycoprotein ELISA Kit, alpha-2-HS-glycoprotein 1 ELISA Kit, alpha-2-HS-glycoprotein L homeolog ELISA Kit, Cphamn1_1981 ELISA Kit, AHSG ELISA Kit, ahsg ELISA Kit, ahsg1 ELISA Kit, ahsg.L ELISA Kit, Ahsg ELISA Kit

Background: Fetuin-A, also known as Alpha-2-HS-glycoprotein (AHSG) is a protein that in humans is encoded by the AHSG gene. This gene spans approximately 8.2 kb and contains 7 exons. Fetuin-A belongs to the fetuin class of plasma binding proteins and is more abundant in fetal than adult blood. It is present in the serum, and synthesized by hepatocytes. It may plays some role in the metabolism of bone because of its high affinity for calcium. Fetuin-A is a potent inhibitor of pathological calcification. In the test tube Fetuin-A can bind multiple ligands and therefore has been claimed to be involved in several functions, such as endocytosis, brain development and the formation of bone tissue.