8-OHDG ELISA Kit

Catalog No. ABIN1020374

Product Details 8-OHDG ELISA Kit

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.5-200 ng/mL

Minimum Detection Limit: 0.5 ng/mL

Application: ELISA

Purpose: This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.

Sample Type: Urine

Analytical Method: Quantitative

Specificity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).

Characteristics: For serum and tissue samples, Highly Sensitive 8-OHdG Check is recommended.

Components: 8-OHdG Microtiter Plate: Precoated with 8-OHdG(12 X 8wells, split type)* 1 plate
Primary Antibody: Anti 8-OHdG antibody, powder. 1 vial
Primary Antibody Solution 1 vial (6mL)
Secondary Antibody: HRP-anti mouse antibody, powder. 1 vial
Secondary Antibody Solution: 1 vial (12mL)
Chromatic Solution: 3,3',5,5'-tetramethylbenzidine. 1 vial (0.25mL)
Diluting Solution: H2O2 containing buffer. 1 vial (12mL)
Washing Solution(5x): 2 vials (26mL x 2)
Reaction Terminating Solution: 1M Phosphoric acid. 1 vial
(12mL) Standard 8-OHdG Solution: Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL). 1 vial each
Plate Seal: 2 sheets

Material not included: Micropipet and chip (100 µL, 1000 µL)
Measuring pipet (10 mL, 20 mL)/ measuring cylinders
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet
Microplate reader (filter, 450 nm).

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for 8-OHDG ELISA Kit (ABIN1020374)

Ishibashi, Sato, Rikitake, Seo, Kurokawa, Hara, Naritomi, Hara, Nagao: "Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis: an open-label pilot study." in: Medical gas research, Vol. 2, Issue 1, pp. 27, (2013) (PubMed).

Kawakatsu, Urata, Goto, Ono, Li: "Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity." in: Journal of radiation research, Vol. 54, Issue 2, pp. 268-76, (2013) (PubMed).

Basavaraj, Vasu Devaraju, Rao: "Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 5, pp. 655-7, (2013) (PubMed).

Kim, Shon, Kim, Kim: "Renal podocyte injury in a rat model of type 2 diabetes is prevented by metformin." in: Experimental diabetes research, Vol. 2012, pp. 210821, (2012) (PubMed).

Cai, Chen, Tao, Guo, Liu, Zheng, Sun, Wang: "Association of base excision repair gene polymorphisms with ESRD risk in a Chinese population." in: Oxidative medicine and cellular longevity, Vol. 2012, pp. 928421, (2012) (PubMed).

Nakajima, Unoda, Ito, Kitaoka, Kimura, Hanafusa: "The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke." in: The open neurology journal, Vol. 6, pp. 51-7, (2012) (PubMed).

Serdar, Lee, Dou: "Biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs) and DNA damage: a cross-sectional pilot study among roofers in South Florida." in: BMJ open, Vol. 2, Issue 4, (2012) (PubMed).

Kawashima, Kawakita, Inaba, Okada, Ito, Shimmura, Watanabe, Shinmura, Tsubota: "Dietary lactoferrin alleviates age-related lacrimal gland dysfunction in mice." in: PLoS ONE, Vol. 7, Issue 3, pp. e33148, (2012) (PubMed).

Pini, Viappiani, Bolla, Masini, Bani: "Prevention of bleomycin-induced lung fibrosis in mice by a novel approach of parallel inhibition of cyclooxygenase and nitric-oxide donation using NCX 466, a prototype cyclooxygenase inhibitor and nitric-oxide donor." in: The Journal of pharmacology and experimental therapeutics, Vol. 341, Issue 2, pp. 493-9, (2012) (PubMed).

Morishita, Watanabe, Hirahara, Akimoto, Muto, Kusano: "Level of 8-OHdG in drained dialysate appears to be a marker of peritoneal damage in peritoneal dialysis." in: International journal of nephrology and renovascular disease, Vol. 5, pp. 9-14, (2012) (PubMed).

Li, Wu, Hsieh, Lu, Tsai, Yu: "Deranged bioenergetics and defective redox capacity in T lymphocytes and neutrophils are related to cellular dysfunction and increased oxidative stress in patients with active systemic lupus erythematosus." in: Clinical & developmental immunology, Vol. 2012, pp. 548516, (2011) (PubMed).

Oka, Goto, Koizumi, Nakamura, Tsuneyama, Zhou, Jo, Fujimoto, Sakurai, Shibahara, Saiki, Shimada: "Effect of Hachimijiogan against Renal Dysfunction and Involvement of Hypoxia-Inducible Factor-1α in the Remnant Kidney Model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 348686, (2011) (PubMed).

Fujiwara, Nakamura, Sato, Kawagoe, Hikichi, Ueda, Node: "Renovascular protective effects of erythropoietin in patients with chronic kidney disease." in: Internal medicine (Tokyo, Japan), Vol. 50, Issue 18, pp. 1929-34, (2011) (PubMed).

Ates, Ulker, Akdemir, Karakaya: "Correlation of Ochratoxin A exposure to urinary levels of 8-hydroxydeoxyguanosine and malondialdehyde in a Turkish population." in: Bulletin of environmental contamination and toxicology, Vol. 86, Issue 3, pp. 258-62, (2011) (PubMed).

Chang, Sha, Zhang, Liu, Rong, Han, Liu, Pan: "The evaluation of the oxidative stress parameters in patients with primary angle-closure glaucoma." in: PLoS ONE, Vol. 6, Issue 11, pp. e27218, (2011) (PubMed).

Tsai, Wu, Cheng, Kao, Kau, Lee, Wei: "Increased response to oxidative stress challenge in Graves' ophthalmopathy orbital fibroblasts." in: Molecular vision, Vol. 17, pp. 2782-8, (2011) (PubMed).

Iskandar, Griffeth, Sharma: "Marijuana-induced mania in a healthy adolescent: a case report." in: General hospital psychiatry, Vol. 33, Issue 6, pp. 640.e3-4, (2011) (PubMed).

Soini, Haapasaari, Vaarala, Turpeenniemi-Hujanen, Kärjä, Karihtala: "8-hydroxydeguanosine and nitrotyrosine are prognostic factors in urinary bladder carcinoma." in: International journal of clinical and experimental pathology, Vol. 4, Issue 3, pp. 267-75, (2011) (PubMed).

Kim, Moon, Park, Lee, Hong: "Changes in oxidative stress biomarker and gene expression levels in workers exposed to volatile organic compounds." in: Industrial health, Vol. 49, Issue 1, pp. 8-14, (2011) (PubMed).

Evans, Olinski, Loft, Cooke: "Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine as a noninvasive biomarker of oxidative stress." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 4, pp. 1249-60, (2010) (PubMed).

Target Details for 8-OHDG

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Abstract: 8-OHDG Products

Target Type: Chemical

Background: About 8-hydroxy-2'-deoxyguanosine (8-OHdG): 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals. This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum , tissue and cultured cells.