8-OHDG ELISA Kit

Catalog No. ABIN1020373

Product Details 8-OHDG ELISA Kit

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.125-10 ng/mL

Minimum Detection Limit: 0.125 ng/mL

Application: ELISA

Purpose: Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum , tissue and cultured cells.

Sample Type: Cell Culture Lysate, Serum, Urine

Analytical Method: Quantitative

Specificity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).

Components: 8-OHdG Microtiter Plate: Precoated with 8-OHdG(12 X 8wells, split type)* 1 plate
Primary Antibody: Anti 8-OHdG antibody, powder. 1 vial
Primary Antibody Solution 1 vial (6mL)
Secondary Antibody: HRP-anti mouse antibody, powder. 1 vial
Secondary Antibody Solution: 1 vial (12mL)
Chromatic Solution: 3,3',5,5'-tetramethylbenzidine. 1 vial (0.25mL)
Diluting Solution: H2O2 containing buffer. 1 vial (12mL)
Washing Solution(5x): 2 vials (26mL x 2)
Reaction Terminating Solution: 1M Phosphoric acid. 1 vial
(12mL) Standard 8-OHdG Solution: Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL). 1 vial each
Plate Seal: 2 sheets

Material not included: Micropipet and chip (100 µL, 1000 µL)
Measuring pipet (10 mL, 20 mL)/ measuring cylinders
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet
Microplate reader (filter, 450 nm).

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for 8-OHDG ELISA Kit (ABIN1020373)

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Target Details for 8-OHDG

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Abstract: 8-OHDG Products

Target Type: Chemical

Background: About 8-hydroxy-2'-deoxyguanosine (8-OHdG): 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals. This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum , tissue and cultured cells.