Neuraminidase Assay Kit

Catalog No. ABIN1000322

Product Details

Target: NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))

Application: Activity Assay (AcA)

Specificity: 0.1 - 10 U/L (colorimetric), 0.01 - 2 U/L (fluorimetric)

Characteristics: Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays.
Simple and convenient.
Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min.
High-throughput. Can be readily automated as a high-throughput 96- well plate assay to screen thousands of samples per day.

Components: Assay Buffer: 6 mL. Substrate: 6 mL. Cofactors: 120 µL. Dye Reagent: 60 µL. Enzyme: 120 µL. Standard: 500 µL.

Material not included: Pipetting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well or 384-well plates (e.g. Corning Costar) and plate reader.

Application Details

Application Notes: Direct Assays: neuraminidase activity in biological samples.
Drug Discovery: evaluation of neuraminidase inhibitors.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

Li, Ma, Sarker, McClane: "CodY is a global regulator of virulence-associated properties for Clostridium perfringens type D strain CN3718." in: mBio, Vol. 4, Issue 5, pp. e00770-13, (2013) (PubMed).

Target Details

Target: NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))

Alternative Name: Neuraminidase (NEU1 Products)

Synonyms: NANH Kit, NEU Kit, SIAL1 Kit, MGC81958 Kit, AA407268 Kit, AA407316 Kit, Aglp Kit, Apl Kit, Bat-7 Kit, Bat7 Kit, G9 Kit, Map-2 Kit, Neu Kit, Neu-1 Kit, neuraminidase 1 Kit, neuraminidase 1 (lysosomal sialidase) L homeolog Kit, NEU1 Kit, neu1.L Kit, neu1 Kit, Neu1 Kit

Target Type: Influenza Protein

Background: Quantitative determination of neuraminidase activity or screening for neuraminidase inhibitor by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure: 60 min.

Neuramindase (also known as Sialidase) is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery. This neuraminidase assay measures the sialic acid released by neuraminidase in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at gamma em/ex = 585/530nm is directly proportional to neuraminidase activity in the sample.