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Chicken IgG isotype control (HRP)

Rockland 003-0302 SDS IgG HRP
Rockland
Catalog No. ABIN964215
Supplier Product No.: 003-0302
  • Target See all IgG products
    IgG
    Host
    • 136
    • 126
    • 118
    • 84
    • 82
    • 55
    • 53
    • 46
    • 44
    • 42
    • 38
    • 33
    • 33
    • 32
    • 24
    • 18
    • 10
    • 8
    • 7
    • 6
    • 4
    • 2
    Chicken
    Conjugate
    HRP
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    003-0302
    Supplier
    Rockland
    Purpose
    Chicken IgG Whole Molecule Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Chicken IgG and anti-Chicken Serum.
    Characteristics
    Chicken IgG peroxidase conjugated, Chicken IgG HRP conjugated, Chicken IgY peroxidase conjugated, Chicken IgY HRP conjugated
    Purification
    This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. 
    Isotype
    IgG
  • Application Notes

    Application Note: Chicken IgG whole molecule peroxidase conjugation has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 1.0 mL

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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