Mouse IgM Isotype Control
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- Target See all IgM products
- IgM
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Host
- Mouse
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Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
- No Cross-Reactivity
- Human, Mouse, Pig, Rat
- Purification
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- Purity
- > 95 % (by SDS-PAGE)
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Clone
- PFR-03
- Isotype
- IgM
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
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