Human IgG isotype control (FITC)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Host
- Human
- Conjugate
- FITC
- Application
- Isotype Control (IsoC), Immunofluorescence (IF)
- Characteristics
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Human IgG protein (Fab'2) (FITC) conjugate
Source: Human serum
Alternative Names: Immunoglobulin G protein (Human) (Fab'2) (FITC) - Purification
- Human IgG protein (Fab'2) (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and dialysis.
- Isotype
- IgG
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized from 0.01 M Na1O4, pH 7.4, with 0.11 NaCl, 10 mg/mL BSA, and 0.01 % thimerisol. Immunoglobulin and protease free.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid repeated freeze/thaw cycles. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen-binding site - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. Treatment with pepsin produces an F(ab)'2 fragment.
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