Mouse IgG1 isotype control (FITC)
-
- Target See all IgG1 products
- IgG1
-
Host
- Mouse
-
Clonality
- Monoclonal
- Conjugate
- FITC
- Application
- Flow Cytometry (FACS), Isotype Control (IsoC), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Characteristics
-
FITC Conjugated Mouse IgG1 Kappa Light Chain Isotype Control antibody
Alternative Names: Monoclonal IgG1 Kappa Light Chain Isotype Control, Anti- IgG1 Kappa Light Chain Isotype Control, IgG1 Kappa Light Chain Isotype Control, Immunoglobulin G1 Kappa Light Chain Isotype Control - Purification
- Affinity purified
- Immunogen
- IgG1 kappa light chain antibody (FITC) was raised in mouse using kapa light chain as the immunogen.
-
Clone
- M12637
- Isotype
- IgG1
-
-
- Application Notes
-
FC: 20 µL per 10^6 cells in 100 µL volume
This immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. - Comment
-
Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, with 0.2 % BSA and 0.09 % NaN3.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
-
Product is photosensitive and should be protected from light.
Do not freeze. - Storage
- 4 °C
- Storage Comment
- Store at 4 °C.
-
- Target
- IgG1
- Abstract
- IgG1 Products
- Synonyms
- IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control
- Target Type
- Antibody
- Background
- The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). A typical antibody is composed of two immunoglobulin heavy chains and two immunoglobulin light chains. There are two types of light chain in humans (as in other mammals), kappa chain, encoded by the immunoglobulin kappa locus on chromosome 2 lambda chain, encoded by the immunoglobulin lambda locus on chromosome 22.
-