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- Target See all IgM products
- IgM
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Host
- Human
- Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), SDS-PAGE (SDS)
- Supplier Product No.
- 009-0107-0001
- Supplier
- Rockland
- Purpose
- Human IgM (myeloma) Whole Molecule
- Cross-Reactivity (Details)
- Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (μ chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross-reactivity will occur with anti-Human IgG.
- Characteristics
- Human immunoglobulin M, Human IgM whole molecule, Human IgM myeloma whole molecule, Hu IgM WM
- Purification
- Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Isotype
- IgM
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Mouse IgM isotype control (FITC)
IsoC, FACS, IF IgM FITC
Mouse IgM isotype controlIsoC, FACS IgM unconjugated
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (APC)IsoC, FACS, IF IgM APC
Mouse IgM isotype control (PE-Cy5)IsoC, FACS, IF IgM PE-Cy5
Mouse IgM isotype control (FITC)IsoC, FACS, IF IgM FITC
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Human IgM whole molecule has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.1 mg/mL
- Buffer
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Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
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