Human IgM Isotype Control
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- Target See all IgM products
- IgM
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Host
- Human
- Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (μ chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross-reactivity will occur with anti-Human IgG.
- Purification
- Human IgM (myeloma) whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Isotype
- IgM
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Human IgM (myeloma) whole molecule can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.1 mg/mL
- Buffer
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Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- IgM
- Abstract
- IgM Products
- Synonyms
- IgM constant region Isotype Control, IGM Isotype Control
- Target Type
- Antibody
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