Rabbit IgG Isotype Control
-
- Target See all IgG products
- IgG
-
Host
- Rabbit
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)
- Supplier Product No.
- 011-0102-0010
- Supplier
- Rockland
- Purpose
- Rabbit IgG Whole Molecule
- Cross-Reactivity (Details)
- Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
- Characteristics
- Rabbit immunoglobulin G
- Purification
- Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Isotype
- IgG
-
Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC IgG unconjugated
-
- Application Notes
-
Immunohistochemistry Dilution: User Optimized
Application Note: Rabbit IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
- Concentration
- 10.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
-
