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- Target See all IgG3 products
- IgG3
- Reactivity
- Llama
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Host
- Llama
- Application
- Flow Cytometry (FACS), ELISA, Western Blotting (WB)
- Purification
- Llama IgG3 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. No bands corresponding to llama IgG1 or IgG2 are observed.
- Isotype
- IgG3
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Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype control (PE-Cy7)FACS, ELISA Monoclonal B10 IgG3 PE-Cy7
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (FITC)FACS, ELISA, IsoC, WB, FM Monoclonal MG3 IgG3 FITC
Human IgG3 isotype controlIsoC Polyclonal IgG3 unconjugated
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- Application Notes
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Flow Cytometry Dilution: User Optimized
Application Note: Llama IgG3 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- Comment
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Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35 % - 70 % of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG3
- Abstract
- IgG3 Products
- Synonyms
- IgG3 Isotype Control, Immunoglobulin heavy constant gamma 3 Isotype Control, immunoglobulin heavy constant gamma 3 (G3m marker) Isotype Control, Ighg3 Isotype Control, IGHG3 Isotype Control
- Target Type
- Antibody
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