Mouse IgG2b isotype control (APC)
(5 references)-
- Target See all IgG2b products
- IgG2b
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Host
- Mouse
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Clonality
- Monoclonal
- Conjugate
- APC
- Application
- Flow Cytometry (FACS)
- Specificity
- This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
- No Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- KLH-coupled trinitrophenol
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Clone
- MPC-11
- Isotype
- IgG2b kappa
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Mouse IgG2b isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin
Mouse IgG2b isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype control (PE)FACS Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (PerCP)FACS Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (FITC)FACS, IsoC, IF IgG2b FITC
Mouse IgG2b isotype controlFACS, IsoC IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS, ELISA Monoclonal A-1 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Comment
-
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." in: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).
: "Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells." in: World journal of gastroenterology, Vol. 16, Issue 5, pp. 618-24, (2010) (PubMed).
: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).
: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, (2006) (PubMed).
: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, (1999) (PubMed).
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: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." in: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).
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- Target
- IgG2b
- Alternative Name
- IgG2b (IgG2b Products)
- Synonyms
- IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
- Target Type
- Antibody
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