Mouse IgG2a isotype control (APC)
(4 references)-
- Target See all IgG2a products
- IgG2a
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Host
- Mouse
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Clonality
- Monoclonal
- Conjugate
- APC
- Application
- Flow Cytometry (FACS)
- Specificity
- This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
- No Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
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Clone
- MOPC-173
- Isotype
- IgG2a kappa
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Mouse IgG2a isotype control (PerCP)
FACS Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (Biotin)FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (FITC)FACS Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (PE)FACS Monoclonal MOPC-173 IgG2a kappa PE
Mouse IgG2a isotype control (FITC)FACS, IsoC, IF IgG2a FITC
Mouse IgG2a isotype controlFACS, IsoC IgG2a unconjugated
Mouse IgG2a isotype control (FITC)FACS, ELISA Monoclonal HOPC-1 IgG2a FITC
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Comment
-
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP." in: Nature protocols, Vol. 9, Issue 2, pp. 337-61, (2014) (PubMed).
: "The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18." in: Blood, Vol. 109, Issue 8, pp. 3513-20, (2007) (PubMed).
: "The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications." in: Annales d'immunologie, Vol. 127, Issue 5, pp. 607-31, (1977) (PubMed).
: "Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 116, Issue 1, pp. 65-74, (1976) (PubMed).
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: "Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP." in: Nature protocols, Vol. 9, Issue 2, pp. 337-61, (2014) (PubMed).
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Synonyms
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Target Type
- Antibody
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