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- Target See all IgM products
- IgM
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Host
- Mouse
- Application
- Isotype Control (IsoC), Flow Cytometry (FACS)
- Characteristics
- This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
- Purification
- Purified
- Isotype
- IgM
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Mouse IgM isotype control
IsoC Monoclonal MML IgM unconjugated
Rat IgM isotype controlIsoC, ELISA, WB IgM Native unconjugated
Mouse IgM isotype controlIsoC Polyclonal IgM unconjugated
Mouse IgM isotype control (FITC)IsoC, FACS, IF IgM FITC
Human IgM isotype controlELISA, WB, IHC IgM unconjugated
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
Rat IgM isotype control (FITC)IsoC, ELISA, WB, IM IgM Native FITC
Mouse IgM isotype control (APC)IsoC, FACS, IF IgM APC
Mouse IgM isotype control (PE-Cy5)IsoC, FACS, IF IgM PE-Cy5
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Procedure
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
- Repeat step 5.
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4,
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- IgM
- Abstract
- IgM Products
- Synonyms
- IgM constant region Isotype Control, IGM Isotype Control
- Target Type
- Antibody
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