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- Target See all IgG2b products
- IgG2b
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Host
- Mouse
- Application
- Flow Cytometry (FACS), Isotype Control (IsoC)
- Characteristics
- This antibody can be used as a mouse IgG2b isotype control in flow cytometry and other applications.
- Purification
- Purified
- Isotype
- IgG2b
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Mouse IgG2b isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin
Mouse IgG2b isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype control (PE)FACS Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (PerCP)FACS Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (APC)FACS Monoclonal MPC-11 IgG2b kappa APC
Mouse IgG2b isotype control (FITC)FACS, IsoC, IF IgG2b FITC
Mouse IgG2b isotype control (FITC)FACS, ELISA Monoclonal A-1 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Procedure
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
- Repeat step 5.
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- IgG2b
- Abstract
- IgG2b Products
- Synonyms
- IgG2b Isotype Control, Igh-3 Isotype Control, gamma2b Isotype Control, immunoglobulin heavy constant gamma 2B Isotype Control, Ighg2b Isotype Control
- Target Type
- Antibody
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