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- Target See all IgG2a products
- IgG2a
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Host
- Mouse
- Application
- Flow Cytometry (FACS), Isotype Control (IsoC)
- Characteristics
- This antibody can be used as a mouse IgG2a isotype control in flow cytometry and other applications.
- Purification
- Purified
- Isotype
- IgG2a
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Mouse IgG2a isotype control (APC)
FACS Monoclonal MOPC-173 IgG2a kappa APC
Mouse IgG2a isotype control (PerCP)FACS Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (Biotin)FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (FITC)FACS Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (PE)FACS Monoclonal MOPC-173 IgG2a kappa PE
Mouse IgG2a isotype control (FITC)FACS, IsoC, IF IgG2a FITC
Mouse IgG2a isotype control (FITC)FACS, ELISA Monoclonal HOPC-1 IgG2a FITC
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Procedure
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
- Repeat step 5.
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Synonyms
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Target Type
- Antibody
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