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Mouse IgM isotype control (PE-Cy5)

IsoC, FACS, IF IgM PE-Cy5
Catalog No. ABIN2145362
  • Target See all IgM products
    IgM
    Host
    • 51
    • 37
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Mouse
    Conjugate
    PE-Cy5
    Application
    Isotype Control (IsoC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Characteristics
    This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
    Isotype
    IgM
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 20 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    IgM
    Abstract
    IgM Products
    Synonyms
    IgM constant region Isotype Control, IGM Isotype Control
    Target Type
    Antibody
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