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- Target See all IgG2a products
- IgG2a
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Host
- Mouse
- Conjugate
- PE-Cy5
- Application
- Flow Cytometry (FACS), Isotype Control (IsoC), Immunofluorescence (IF)
- Characteristics
- This antibody can be used as a mouse IgG2a isotype control in flow cytometry and other applications.
- Isotype
- IgG2a
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Mouse IgG2a isotype control (APC)
FACS Monoclonal MOPC-173 IgG2a kappa APC
Mouse IgG2a isotype control (PerCP)FACS Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (Biotin)FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (FITC)FACS Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (PE)FACS Monoclonal MOPC-173 IgG2a kappa PE
Mouse IgG2a isotype control (FITC)FACS, IsoC, IF IgG2a FITC
Mouse IgG2a isotype controlFACS, IsoC IgG2a unconjugated
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Procedure
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 20 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Synonyms
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Target Type
- Antibody
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