Histone H4ac antibody (N-Term)
HIST1H4C
Reactivity: Human, Mouse
ChIP-seq, ChIP, DB, ICC, IF, IHC, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- Histone H4ac (HIST1H4C) (Histone Cluster 1 H4 Family Member c (HIST1H4C))
- Binding Specificity
- N-Term
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Characteristics
- Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation. Histone H4ac (pan-acetyl) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
- Purification
- Protein A Chromatography
- Immunogen
- This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena hv1, an H2A variant with homology to mammalian histone H4. The antibody recognizes acetylated histone H4, but not H2A, in HeLa extracts.
- Isotype
- IgG
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Buffer
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
-
- Target
- Histone H4ac (HIST1H4C) (Histone Cluster 1 H4 Family Member c (HIST1H4C))
- Alternative Name
- Histone H4ac
- Molecular Weight
- 8 kDa
- NCBI Accession
- XP_001016593
-
You are here:
