Hba-x Protein (AA 1-142) (His tag)
HBA-X
Origin: Human
Host: Baculovirus infected Insect Cells
Recombinant
> 85 % by SDS - PAGE
SDS
1 image
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- Target
- Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-142
- Origin
- Human
- Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Hba-x protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH
- Purity
- > 85 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 50 mM Tris-HCl ( pH 7.5) containing 30 % glycerol, 0.5M NaCl, 2 mM DTT, 0.1 mM PMSF
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))
- Alternative Name
- Hba-x
- Synonyms
- AI450015 Protein, Hbz Protein, hemoglobin X, alpha-like embryonic chain in Hba complex Protein, Hba-x Protein
- Background
- RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 60.2kDa (533aa) 70-100kDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_003152
- UniProt
- P23443
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