H2AFB2 Protein (Myc-DYKDDDDK Tag)
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- Target
- H2AFB2 (H2A Histone Family, Member B2 (H2AFB2))
- Protein Type
- Recombinant
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This H2AFB2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Histone H2A-Bbd type 2/3 / H2AFB2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- H2AFB2 (H2A Histone Family, Member B2 (H2AFB2))
- Alternative Name
- Histone h2a-Bbd Type 2/3,h2afb2
- Synonyms
- H2A.Bbd Protein, EG624153 Protein, H2A.Bbd4 Protein, H2afb2 Protein, H2A histone family member B2 Protein, H2A histone family, member B2 Protein, H2AFB2 Protein, H2afb2 Protein
- Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the middle copy.
- Molecular Weight
- 12.5 kDa
- NCBI Accession
- NP_001017991
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