HA-Tag Products
(HA-Tag)
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Antibodies that specifically recognize and bind to the HA tag (hemagglutinin tag) are widely used in molecular biology and biochemistry research. The HA tag is a small protein sequence derived from the influenza virus hemagglutinin protein. Researchers often fuse this tag to the target protein of interest to facilitate its detection, purification, and characterization. Here are some common applications of antibodies that detect the HA tag:
- Protein Detection: HA-tagged proteins are often expressed in cells or organisms to study their function and localization. Antibodies against the HA tag can be used to detect the presence of the tagged protein in various assays such as Western blotting, immunoprecipitation, and immunofluorescence.
- Protein Localization: By attaching an HA tag to a protein, researchers can study its cellular localization using immunofluorescence or immunohistochemistry techniques. Antibodies against the HA tag enable visualization of the protein within cells and tissues.
- Protein Purification: The HA tag is commonly used as an affinity tag for protein purification. Researchers can use antibody affinity chromatography to purify the HA-tagged protein from a complex mixture, exploiting the specific interaction between the tag and the antibody.
- Protein-Protein Interactions: Antibodies against the HA tag can be used in co-immunoprecipitation experiments to study protein-protein interactions. If one protein is tagged with HA and another with a different tag, the interaction can be detected by co-immunoprecipitating both proteins using their respective antibodies.
- Protein Tracking: In live-cell imaging studies, HA-tagged proteins can be tracked over time using fluorescence microscopy. The specific binding of the HA tag antibody allows researchers to monitor the movement and dynamics of the tagged protein within living cells.
- Flow Cytometry: HA-tagged proteins expressed on the surface of cells can be detected using flow cytometry. Antibodies against the HA tag, labeled with fluorescent markers, enable quantitative analysis of the tagged protein's expression levels on individual cells.
- Enzyme-Linked Immunosorbent Assay (ELISA): HA-tagged proteins can be quantified using ELISA assays. An immobilized antibody against the HA tag captures the protein of interest, and a second antibody, often labeled with an enzyme, is used for detection and quantification.
- Virus-Like Particle Studies: The HA tag can be used in the context of virus-like particles (VLPs) to study aspects of virus assembly and budding. Antibodies against the HA tag can be used to detect and analyze these VLPs.
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Latest Publications for our HA-Tag products
: "Microcontact Printing of Biomolecules on Various Polymeric Substrates: Limitations and Applicability for Fluorescence Microscopy and Subcellular Micropatterning Assays." in: ACS applied polymer materials, Vol. 4, Issue 10, pp. 6887-6896, (2022) (PubMed).: "The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair." in: Developmental cell, Vol. 56, Issue 23, pp. 3192-3202.e8, (2021) (PubMed).
: "AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody." in: PLoS genetics, Vol. 17, Issue 8, pp. e1009757, (2021) (PubMed).
: "Highly Modular Protein Micropatterning Sheds Light on the Role of Clathrin-Mediated Endocytosis for the Quantitative Analysis of Protein-Protein Interactions in Live Cells." in: Biomolecules, Vol. 10, Issue 4, (2021) (PubMed).
: "MARCH8 inhibits viral infection by two different mechanisms." in: eLife, Vol. 9, (2020) (PubMed).
: "The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition." in: eLife, Vol. 9, (2020) (PubMed).
: "Anterograde trafficking signals in GABAA subunits are required for functional expression." in: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).
: "Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication." in: Emerging microbes & infections, Vol. 8, Issue 1, pp. 1300-1313, (2020) (PubMed).
: "Planar-Polarized Semaphorin-5c and Plexin A Promote the Collective Migration of Epithelial Cells in Drosophila." in: Current biology : CB, Vol. 29, Issue 6, pp. 908-920.e6, (2020) (PubMed).
: "Chemogenetic Activation of Excitatory Neurons Alters Hippocampal Neurotransmission in a Dose-Dependent Manner." in: eNeuro, Vol. 6, Issue 6, (2020) (PubMed).
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