Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

FeSOD antibody

FeSOD Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Zea mays WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2749232
  • Target
    FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
    Reactivity
    Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Zea mays
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    No cross-reactivity with: cyanobacteria
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 25 / 22 kDa
    Purification
    serum
    Immunogen
    overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
  • Application Notes
    Recommended Dilution: 1 : 1500 to 1 : 5000 with alkaline phosphatase (WB).
    Comment

    The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 µM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µL of sterile water.
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
  • Page, Allen, Kropat, Urzica, Karpowicz, Hsieh, Loo, Merchant: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).

    Abdel-Ghany, Müller-Moulé, Niyogi, Pilon, Shikanai: "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts." in: The Plant cell, Vol. 17, Issue 4, pp. 1233-51, (2005) (PubMed).

  • Target
    FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
    Background
    AGI Code: At4g25100
    Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
    Molecular Weight
    expected: 25 kDa, apparent: 22 kDa
    UniProt
    A8IGH1
You are here:
Support