D1-Nt antibody
PsbA
Reactivity: Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum
WB
Host: Rabbit
Polyclonal
unconjugated
1 image
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- Target
- D1-Nt (PsbA)
- Reactivity
- Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Specificity
- Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.
- Immunogen
- synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.
- Isotype
- IgG
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- Application Notes
- Western blot (1 : 2.000 for ECL)
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C
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- Target
- D1-Nt (PsbA)
- Background
- D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).
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