Cyclin L1/L2 antibody
(1 reference)-
- Target
- Cyclin L1/L2 (CCNL1/2)
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- Cross-Reactivity
- Human, Mouse (Murine), Rat (Rattus)
- Purification
- The product was purified from monospecific antiserum by Protein A chromatography. This antibody will react with all forms of Cyclin L b (cyclin L1b and cyclin L2b). Cross reactivity with Cyclin L beta proteins from other sources is unknown.
- Immunogen
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Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic construct consisting of full length Human Cyclin L1b protein.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 2 mg/mL - 20 μg/mL
Application Note: This Protein A purified antibody has been tested for use in ELISA, western blot, immunohistochemistry, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 25 kDa to 35 kDa in size corresponding to Cyclin L1b and Cyclin L2b by western blotting in the appropriate cell lysate or extract.
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:25,000
Western Blot Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).
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: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).
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- Target
- Cyclin L1/L2 (CCNL1/2)
- Alternative Name
- Cyclin L1/L2
- Background
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Synonyms: rabbit anti-Cyclin L1/L2 Antibody, rabbit anti-Cyclin L1 Antibody, rabbit anti-Cyclin L2 Antibody, Cyclin L beta, CCNL1, CCNL2, Paneth cell-enhanced expression protein
Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.
Gene Name: CCNL1, CCNL2
- Gene ID
- 57018
- NCBI Accession
- NP_064703
- UniProt
- Q9UK58
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