Library Generation & Screening

Yeast & Phage Display, Antibody Expression

The combined expertise of antibodies-online and Rockland in antibody discovery now includes antibody display technology. Discover your ideal antibody using yeast or phage antibody display libraries generated from immunization campaigns using various animal species, derived from naïve animals (i.e., non-immunized host), or synthetic antibody libraries.

Antibody Display Libraries

Libraries can be made from hosts that include mouse, rabbit, alpaca or llama, and human. Construction of the library is generated with antibody genes derived from sources that may include blood (i.e., PBMCs), spleen, bone marrow, or other tissues as appropriate for your project. The library is then screened for the binding specificity of interest. This is typically a biotinylated form of the target, but other screening methodologies can also be used such as panning on solid surfaces (e.g., coated plates) or on cells and even tissues. Selected antibody clones are sequenced, then the gene is synthesized in the format of interest, and the purified recombinant antibody proteins produced for further use.