No significant difference in binding to Factor VII and Factor VIIa
Cross-Reactivity (Details)
Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity
primates
Characteristics
Expected / apparent Molecular Weight of the Antigene: 52 kDa (this is MW of Factor VIIa)
Purification
affinity purified
Immunogen
Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk A/S Denmark)
Application Notes
1: 15 000 (I-ELISA), 14 μg/mL (IL)
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 μL of sterile water
Buffer
PBS pH 7.4
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Storage Comment
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them, to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Target
Coagulation Factor VIIa (FVIIa)
Background
Factor VIIa (FVIIa) is a key serine protease involved in the initiation of the coagulation cascade. FVIIa requires tissue factor (TF), a membrane bound protein, as an essential cofactor for maximal activity towards its biological substrates Factor X, Factor IX and Factor VII (FVII).