Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity
Chlamydomonas reinhardtii
Characteristics
Expected / apparent Molecular Weight of the Antigene: 24 / 27-28 kDa (SDS PAGE)
Purification
serum
Immunogen
mature CGE1 protein of Chlamydomonas reinhardtii, UniProt: Q945T1, expressed with N-terminal hexahistidin tag in E. coli, purified with Ni-NTA
Application Notes
1:10 000 with standard ECL (WB)
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 100 μL of sterile water.
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Storage Comment
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Target
CGE1
(Chloroplast GrpE Protein (CGE1))
Alternative Name
CGE1
Background
CGE1 is a nuclear-encoded, chloroplast-targeted co-chaperone of chloroplast HSP70B. It forms dimers that interact with HSP70B in the ADP-state.