CD11R1 antibody
CD11R1
Reactivity: Pig, Guinea Pig, Human
FACS, IP, IHC (fro)
Host: Mouse
Monoclonal
MIL4
unconjugated
-
- Target
- CD11R1
- Reactivity
- Pig, Guinea Pig, Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognizes the cell surface antigen classified as CD11R1 and stains Porcine eosinophils, a subset of neutrophils and NK cells. The CD11R1 antigen is a 165kD molecule, identical in size to Human CD11b. However, the expression pattern of CD11b in the Human and CD11R1 in the pig are different. Clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994). Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of 95 kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of 165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001). Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980). Species: Human, Pig, Guinea Pig.
- Purification
- Affinity Chromatography on Protein G
- Immunogen
- Porcine Lamina Propria Leucocytes
- Clone
- MIL4
- Isotype
- IgG1
-
- Application Notes
- Flow Cytometry: Use 10 μL of 1/25-1/200 diluted antibody to label 1x10 6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- PBS Preservatives: 0.09 % Sodium Azide Purity: Tissue Culture Supernatant containing 0.2M Tris/HCl pH 7.4 and 5-10 % foetal calf serum
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- Expiry Date
- 12 months
-
- Target
- CD11R1
- Alternative Name
- cd11r1
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