Carboxylesterase 1G (CES1G) antibody
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- Target
- Carboxylesterase 1G (CES1G)
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Immunoprecipitation (IP), Western Blotting (WB)
- Purification
- This product was prepared from monospecific antiserum by delipidation and defibrination. The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie. IL-1 stimulated hCE1 production from fibroblasts).
- Immunogen
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Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.
Immunogen Type: Recombinant Protein
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- Application Notes
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Application Note: This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract. In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Western Blot Dilution: 1:2,500 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- Carboxylesterase 1G (CES1G)
- Alternative Name
- Liver Carboxylesterase-1
- Synonyms
- ACAT antibody, CE-1 antibody, CEH antibody, CES2 antibody, HMSE antibody, HMSE1 antibody, PCE-1 antibody, REH antibody, SES1 antibody, TGH antibody, hCE-1 antibody, Ces-1 antibody, Ces1 antibody, Ses-1 antibody, carboxylesterase 1 antibody, carboxylesterase 1G antibody, liver carboxylesterase antibody, similar to esterase 2 antibody, CES1 antibody, Ces1g antibody, LOC100009551 antibody, LOC684016 antibody
- Background
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Synonyms: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH
Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.
Gene Name: CES1
- Gene ID
- 1066, 68508965
- UniProt
- P23141
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