TMB ELISA Peroxidase Substrate

Catalog No. ABIN925629

Product Details

Application: ELISA

Characteristics: TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow.
Synonyms: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE

Application Details

Application Notes: TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 X

Storage: 4 °C