Bovine Serum Albumin (30% Solution)
RIA
30% (w/v)
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- Reactivity
- Cow
- Application
- Radioimmunoassay (RIA)
- Characteristics
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Concentration Definition: by dry weight
Blocker Type: BSA
Type: Blocking Reagent
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
ELISA Coating StabilizerELISA 1 X
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- Application Notes
- BOVINE SERUM ALBUMIN 30% Solution is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody, and use as a blocking agent to reduce non-specific binding.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 30% (w/v)
- Buffer
- 0.85% (w/v) Sodium Chloride
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Background
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Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Synonyms: BOVINE SERUM ALBUMIN, BSA, BSA Blocker, BSA Blocking, BSA30
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