Blotto Immunoanalytical Grade (Non-Fat Dry Milk)

Catalog No. ABIN925606

Product Details

Application: Blocking Reagent (BR), Dot Blot (DB), Western Blotting (WB)

Characteristics: Supplier produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.

Application Details

Application Notes: Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.  BLOTTO A is a convenient general purpose blocking agent.  BLOTTO B is recommended for blocking when phospho-specific antibodies are used.  BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025).  Users should consider the addition of protease inhibitors and phosphatase inhibitors when required.  Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Storage: -20 °C